先后承担国家重点研发计划项目，国家“973”计划，国家自然科学基金等课题40余项，近五年代表性项目如下：

1)国家重点研发计划项目：猪初始态（naive）多能干细胞系建立及多能性调控机制解析（2015-2020）

2)国家自然科学基金面上项目：NANOG在猪早期胚胎和多能性干细胞中物种特异性转录表达模式与功能机制研究（2023-2026）

3)国家自然科学基金面上项目：OCT4在猪滋养层发育中的功能及作用网络（2019-2022）

4)黑龙江省自然科学基金重点项目：猪高效基因编辑体系创建及应用研究（2022-2025） 

1)Bou, G., Guo, J., Liu, S., Guo, S., Davaakhuu, G., Lv, Q., Xue, B., Qiao, S., Lv, J., Weng, X., Zhao, J., Zhang, Y., He, Y., Zhang, H., Chai, Z., Liu, Y., Yu, Y., Qu, B., Sun, R., Shen, X.,Liu, Z*. . OCT4 expression transactivated by GATA protein is essential for non-rodent trophectoderm early development. Cell reports 2022, 41(8), 111644. (IF: 9.955, 1区TOP)

2) Zhi M, Zhang J, Tang Q, Yu D, Gao S, Gao D, Liu P, Guo J, Hai T, Gao J, Liu Z*, Zheng C*, Li M*, Han J*: Generation and characterization of stable pig pregastrulation epiblast stem cell lines. Cell Res 2022, 32:383-400. (IF: 46.297, 1区TOP)

3) Yu Z, Zhou S, Liu M, Chen L, Zhang X, Yang X, Cui H, Jiang C, Lv Y, Yan T, Liu Z*: Porcine pluripotent stem cells established from LCDM medium with characteristics differ from human and mouse extended pluripotent stem cells. Stem Cells 2022. (IF: 5.845, 2区)

4) Zhang YT, Liu Y, Liang HL, Xu QQ, Liu ZH*, Weng XG*: Metabolomic differences of seminal plasma between boars with high and low average conception rates after artificial insemination. Reprod Domest Anim 2021, 56:161-171. (IF: 1.858, 3区)

5) Li X, Yu Y, Wei R, Li Y, Lv J, Liu Z*, Zhang Y*: In vitro and in vivo study on angiogenesis of porcine induced pluripotent stem cell-derived endothelial cells. Differentiation 2021, 120:10-18. (IF: 3.533, 3区)

6) Bou G, Guo J, Fang Y, Li X, Wei R, Li Y, Liu Z*: Interspecies cell fusion between mouse embryonic stem cell and porcine pluripotent cell. Reprod Domest Anim 2021, 56:1095-1103. (IF: 1.858, 3区)

7) Weng XG, Cai MM, Zhang YT, Liu Y, Liu C, Liu ZH*: Improvement in the in vitro development of cloned pig embryos after kdm4a overexpression and an H3K9me3 methyltransferase inhibitor treatment. Theriogenology 2020, 146:162-170. (IF: 2.923, 2区TOP)

8) Li Y, Wu S, Li X, Guo S, Cai Z, Yin Z, Zhang Y, Liu Z*: Wnt signaling associated small molecules improve the viability of pPSCs in a PI3K/Akt pathway dependent way. J Cell Physiol 2020, 235:5811-5822. (IF: 6.513, 2区)

9) Kong Q, Yang X, Zhang H, Liu S, Zhao J, Zhang J, Weng X, Jin J, Liu Z*: Lineage specification and pluripotency revealed by transcriptome analysis from oocyte to blastocyst in pig. FASEB J 2020, 34:691-705. (IF: 5.834, 2区)

10) Zhang X, Xue B, Li Y, Wei R, Yu Z, Jin J, Zhang Y, Liu Z*: A novel chemically defined serum- and feeder-free medium for undifferentiated growth of porcine pluripotent stem cells. J Cell Physiol 2019. (IF: 6.513, 2区)

11) Weng XG, Liu Y, Zhou SH, Zhang YT, Shao YT, Xu QQ, Liu ZH*: Evaluation of porcine circovirus type 2 infection in in vitro embryo production using naturally infected oocytes. Theriogenology 2019, 126:75-80. (IF: 2.923, 2区TOP)

12) Lv J, Wu S, Wei R, Li Y, Jin J, Mu Y, Zhang Y, Kong Q, Weng X, Liu Z*: The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells. J Vet Sci 2019, 20:e23. (IF: 1.603, 3区)

13) Kong QR, Zhang JM, Zhang XL, Zong M, Zheng KL, Liu L, Meng FX, Zhang TT, Weng XG, Mu YS*, Liu ZH*: Endo-siRNAs repress expression of SINE1B during in vitro maturation of porcine oocyte. Theriogenology 2019, 135:19-24. (IF: 2.923, 2区TOP)

14) Kong Q, Quan X, Du J, Tai Y, Liu W, Zhang J, Zhang X, Mu Y*, Liu Z*: Endo-siRNAs regulate early embryonic development by inhibiting transcription of long terminal repeat sequence in pigdagger. Biol Reprod 2019, 100:1431-1439. (IF: 4.161, 3区)

15) Guo S, Liu S, Bou G, Guo J, Jiang L, Chai Z, Cai M, Mu Y, Liu Z*: Fetal bovine serum promotes the development of in vitro porcine blastocysts by activating the Rho-associated kinase signalling pathway. Reprod Fertil Dev 2019, 31:366-376. (IF: 1.973, 4区)

1) 牟彦双，李佳男，刘忠华，姜超前，王玥，矫彦文 (一种附加型CRISPR/Cas9表达载体及其构建方法与应用). 专利授权号：ZL201910212739.1，中国，2022年。

2) 吕嘉伟，赵艳华，刘忠华（一种通过酶切基因组构建CRISPR/Cas9基因组敲除文库的方法）. 专利授权号：ZL201710463397.1，中国，2018年。

3) 翁晓刚，牟彦双，张宇霆，蔡铭铭，宋键，刘忠华（一种猪冻精解冻稀释液及其配制方法）. 专利授权号：ZL201710802837.1，中国，2018年。

4) 刘忠华，郇延军，牟彦双，孔庆然，朱江，吕明（一种提高体外受精胚胎发育率的方法）. 专利授权号：ZL201310608158.2，中国，2018年。

5) 孔庆然，解炳腾，刘忠华（一种提高猪细胞重编程能力的方法及应用）. 专利授权号：ZL201410613163.7，中国，2017年。