
课程编号 | 课程名称 | 课程级别 | 学时 |
1、国家重点研发计划项目子任务:多基因编辑系统的构建和验证(2022-2026)
2、黑龙江省自然科学基金联合项目:IGFBP3基因调控猪脂肪细胞增殖分化机制研究(2020-2023)
3、农业部转基因生物新品种培育重大专项课题子任务:动物重要基因克隆及功能验证(2016-2020)
1.Jiang, C., Geng, L., Wang, J., Liang, Y., Guo, X., Liu, C., Zhao, Y.,
Jin, J., Liu, Z., & Mu, Y. (2023). Multiplexed Gene Engineering Based on
dCas9 and gRNA-tRNA Array Encoded on Single Transcript. International journal of molecular sciences, 24(10), 8535.
2.Fan, J., Liu, C., Zhao, Y., Xu, Q., Yin, Z., Liu, Z., & Mu, Y.
(2024). Single-Cell RNA Sequencing Reveals Differences in Chromatin Remodeling
and Energy Metabolism among In Vivo-Developed, In Vitro-Fertilized, and
Parthenogenetically Activated Embryos from the Oocyte to 8-Cell Stages in Pigs. Animals(Basel), 14(3), 465.
3.Guo, X., Liu, C., Zhao, Y., Jiang, C., Jin, J.,
Liu, Z., & Mu, Y. (2024). CRISPR Ribonucleoprotein-Mediated Precise Editing
of Multiple Genes in Porcine Fibroblasts. Animals(Basel), 14(4), 650.
4.Zhao, Y., Li, X., Liu, C., Jiang, C., Guo, X., Xu, Q., Yin, Z., Liu, Z.,
& Mu, Y. (2024). Improving the Efficiency of CRISPR
Ribonucleoprotein-Mediated Precise Gene Editing by Small Molecules in Porcine
Fibroblasts. Animals(Basel), 14(5), 719.
5.Guo, X., Geng, L., Jiang, C., Yao, W., Jin, J., Liu, Z., & Mu, Y.
(2023). Multiplexed genome engineering for porcine fetal fibroblasts with
gRNA-tRNA arrays based on CRISPR/Cas9. Animal biotechnology, 34(9), 4703–4712.
6.Liang, Y., Wang, J., Li, X., Wu, S., Jiang, C., Wang, Y., Li, X., Liu,
Z. H., & Mu, Y. (2022). Exploring differentially expressed genes related to
metabolism by RNA-Seq in porcine embryonic fibroblast after insulin treatment. Journal of veterinary science, 23(6), e90.
7.Kong, Q. R., Zhang, J. M., Zhang, X. L., Zong, M., Zheng, K. L., Liu,
L., Meng, F. X., Zhang, T. T., Weng, X. G., Mu, Y. S., & Liu, Z. H. (2019).
Endo-siRNAs repress expression of SINE1B during in vitro maturation of
porcine oocyte. Theriogenology, 135, 19–24.
8.Li, X. , Yu, T. ,
Li, M. , Wang, Y. , Meng, B. , & Mu, Y. . (2019). Nanog improves type i
collagen expression in human fetal scleral fibroblasts. Archives of Biological Sciences,71,63-70.
9.Mu, Y., Sun, Y., Leng, L., Cao, Z., Li, Y., Li,
H., & Wang, Y. (2019). Effect of IGFBP2 Overexpression on the Expression of
Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes. The journal of poultry science, 56(3), 177–185.
10.Yin, Z.; Zhang, X.; Li, J.;
Jiao, Y.; Kong, Q.; Mu, Y. (2019). Identification of Imprinted Genes and Their
Differentially Methylated Regions in Porcine. Russ. J. Genet. 55,
1488-1498.
11.
王玥; 牟彦双;
刘忠华. 基于CRISPR/Cas系统的单碱基编辑技术研究进展. 中国生物工程杂志 2020, 40, 58-66.
(1) 牟彦双; 李佳男; 刘忠华; 姜超前; 王玥; 矫彦文 ; 一种附加型CRISPR/Cas9表达载体及其构建方法
与应用, 2022-10-11, 中国, ZL201910212739.1
(2) 翁晓刚; 牟彦双; 张宇霆; 蔡铭铭; 宋健; 刘忠华 ; 一种猪冻精解冻稀释液及其配制方法, 2018-11- 02, 中国, ZL201710802837.1
(3) 刘忠华; 郇延军; 牟彦双; 孔庆然; 朱江; 吕明 ; 一种提高体外受精胚胎发育率的方法, 2018-06- 26, 中国, ZL201310608158.2