姓名:牟彦双
性别:男
出生年月:1980-02-06
职称:副研究员
办公电话:
工作单位:东北农业大学
E-mail:muyanshuang@126.com
开设研究生课程情况
课程编号 课程名称 课程级别 学时
近五年主持科研项目概况

1、国家重点研发计划项目子任务:多基因编辑系统的构建和验证(2022-2026)

2、黑龙江省自然科学基金联合项目:IGFBP3基因调控猪脂肪细胞增殖分化机制研究(2020-2023)

3、农业部转基因生物新品种培育重大专项课题子任务:动物重要基因克隆及功能验证(2016-2020)


近五年项目获奖概况



近五年代表性著作、论文

1.Jiang, C., Geng, L., Wang, J., Liang, Y., Guo, X., Liu, C., Zhao, Y., Jin, J., Liu, Z., & Mu, Y. (2023). Multiplexed Gene Engineering Based on dCas9 and gRNA-tRNA Array Encoded on Single Transcript. International journal of molecular sciences24(10), 8535.

2.Fan, J., Liu, C., Zhao, Y., Xu, Q., Yin, Z., Liu, Z., & Mu, Y. (2024). Single-Cell RNA Sequencing Reveals Differences in Chromatin Remodeling and Energy Metabolism among In Vivo-Developed, In Vitro-Fertilized, and Parthenogenetically Activated Embryos from the Oocyte to 8-Cell Stages in Pigs. Animals(Basel)14(3), 465. 

3.Guo, X., Liu, C., Zhao, Y., Jiang, C., Jin, J., Liu, Z., & Mu, Y. (2024). CRISPR Ribonucleoprotein-Mediated Precise Editing of Multiple Genes in Porcine Fibroblasts.  Animals(Basel)14(4), 650.

4.Zhao, Y., Li, X., Liu, C., Jiang, C., Guo, X., Xu, Q., Yin, Z., Liu, Z., & Mu, Y. (2024). Improving the Efficiency of CRISPR Ribonucleoprotein-Mediated Precise Gene Editing by Small Molecules in Porcine Fibroblasts. Animals(Basel)14(5), 719.

5.Guo, X., Geng, L., Jiang, C., Yao, W., Jin, J., Liu, Z., & Mu, Y. (2023). Multiplexed genome engineering for porcine fetal fibroblasts with gRNA-tRNA arrays based on CRISPR/Cas9. Animal biotechnology34(9), 4703–4712.

6.Liang, Y., Wang, J., Li, X., Wu, S., Jiang, C., Wang, Y., Li, X., Liu, Z. H., & Mu, Y. (2022). Exploring differentially expressed genes related to metabolism by RNA-Seq in porcine embryonic fibroblast after insulin treatment. Journal of veterinary science23(6), e90.

7.Kong, Q. R., Zhang, J. M., Zhang, X. L., Zong, M., Zheng, K. L., Liu, L., Meng, F. X., Zhang, T. T., Weng, X. G., Mu, Y. S., & Liu, Z. H. (2019). Endo-siRNAs repress expression of SINE1B during in vitro maturation of porcine oocyte. Theriogenology135, 19–24.

8.Li, X. ,  Yu, T. ,  Li, M. ,  Wang, Y. ,  Meng, B. , &  Mu, Y. . (2019). Nanog improves type i collagen expression in human fetal scleral fibroblasts. Archives of Biological Sciences,71,63-70.

9.Mu, Y., Sun, Y., Leng, L., Cao, Z., Li, Y., Li, H., & Wang, Y. (2019). Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Cultured Chicken Hepatocytes. The journal of poultry science56(3), 177–185.

10.Yin, Z.; Zhang, X.; Li, J.; Jiao, Y.; Kong, Q.; Mu, Y. (2019). Identification of Imprinted Genes and Their Differentially Methylated Regions in Porcine. Russ. J. Genet. 55, 1488-1498.

11.   王玥; 牟彦双; 刘忠华. 基于CRISPR/Cas系统的单碱基编辑技术研究进展. 中国生物工程杂志 2020, 40, 58-66.


近五年授权的专利等情况

(1) 牟彦双; 李佳男; 刘忠华; 姜超前; 王玥; 矫彦文 ; 一种附加型CRISPR/Cas9表达载体及其构建方法 与应用, 2022-10-11, 中国, ZL201910212739.1

 (2) 翁晓刚; 牟彦双; 张宇霆; 蔡铭铭; 宋健; 刘忠华 ; 一种猪冻精解冻稀释液及其配制方法, 2018-11- 02, 中国, ZL201710802837.1

 (3) 刘忠华; 郇延军; 牟彦双; 孔庆然; 朱江; 吕明 ; 一种提高体外受精胚胎发育率的方法, 2018-06- 26, 中国, ZL201310608158.2


其他获奖及荣誉称号